Medical test · Genetic & molecular

Genetic testing

Also known as: DNA test, gene panel, genomic testing

Reading part or all of a person's DNA (from blood or saliva) or a tumour's DNA (from a biopsy) to find variants that cause disease, raise risk, or predict which drug will work. Modern sequencing panels test hundreds of genes at once.

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Abbreviations
NGS, WGS
Kind
Genetic or molecular test
Categories
Genetic & molecular
Specimen
Venous blood, Saliva, Tissue
Method
DNA sequencing, PCR (including RT-PCR and qPCR), Karyotype, Chromosomal microarray
Molecular scope
the field in general

What it measures

MeasureWhat it tells you
Diagnostic testingConfirms a suspected inherited condition (cystic fibrosis, Huntington disease, familial hypercholesterolaemia).
Predictive testingFinds high-risk variants before disease appears (BRCA1/2 for breast and ovarian cancer, Lynch syndrome for bowel cancer).
Tumour profilingMutations in the cancer itself (EGFR, ALK, KRAS in lung cancer) that match targeted drugs.
PharmacogenomicsVariants that change how drugs are handled (DPYD before fluorouracil, HLA-B*57:01 before abacavir).

Why it is ordered

  • A family history of cancer at young ages or in several relatives
  • A child with developmental problems or an unexplained syndrome
  • Choosing targeted cancer treatment
  • Planning a pregnancy in carriers of recessive conditions
  • Sudden cardiac death in a young relative

How it is done

A blood or saliva sample after genetic counselling; results take weeks. Tumour testing uses the existing biopsy.

Preparation. Counselling before predictive tests, because results affect relatives and insurance in some countries.

Reading the result

FindingUsual meaning
Pathogenic variant foundDiagnosis confirmed or high risk established; cascade testing offered to relatives
Variant of uncertain significanceCannot be interpreted yet; not used for clinical decisions
No variant foundLowers but does not abolish risk; the test only reads the genes on the panel

Reference ranges vary by laboratory, method, age, sex and clinical context; a result is read against the person, not a table.

Limitations

Most common diseases are polygenic and environmental, so a single gene test says little; incidental findings and implications for family members need careful counselling.

Catalogued concepts this page covers

Concepts of the master test taxonomy that resolve to this page; each links to its category.

Educational content. Reference ranges differ between laboratories, methods, ages and sexes, and results are always read against the person, the question asked and other findings. This page cannot interpret an individual result.